by BlastRoom
How one DNA molecule becomes two identical ones, what each enzyme does at the fork, and why replication is called semiconservative.
#biology

20 questions
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At a replication fork, one enzyme moves ahead of the copying machinery and breaks the hydrogen bonds between paired bases so the two strands come apart. Which enzyme does this?
Which enzyme travels along an exposed single strand and builds a new DNA strand, adding free nucleotides one at a time and pairing each with the base it faces?
On the lagging strand the new DNA is built as short separate pieces. One enzyme then joins those pieces by sealing the breaks in the sugar-phosphate backbone. Which enzyme is this?
One enzyme in the cell builds a strand whose nucleotides contain ribose and uracil instead of deoxyribose and thymine. Which enzyme is it?
As it builds new DNA during replication, one enzyme can back up, remove a nucleotide it has just paired with the wrong base, and put the correct one in its place. Which enzyme has this proofreading ability?
Bacteria are grown until both strands of every DNA molecule carry a heavy nitrogen label. The cells are moved to unlabeled medium and allowed to copy their DNA once. What does each resulting DNA molecule contain?
Replication is described as semiconservative. What is conserved in each of the two molecules it produces?
Suppose replication worked the way an early competing model proposed: the parent double helix is read but never taken apart. After one round, what would the parent molecule contain?
A skin cell divides many times over a person's life, and its DNA is copied before every division. Why does the genetic information stay the same through all those copies?
During replication a parent strand reading 3'-ATGCCA-5' is used as a template. Which strand is built against it?
A template strand reads 3'-CGATTC-5'. Which sequence does DNA polymerase build along it?
One strand of a DNA molecule reads 5'-AACGTT-3'. What does its partner strand read?
A sample of double-stranded DNA is analyzed and 30% of its bases are found to be adenine. What percentage of its bases is cytosine?
Which sequence of events describes what happens at one replication fork?
On the lagging strand, new DNA appears first as short Okazaki fragments. Which order of events turns them into one continuous strand?
How do the actions of helicase and DNA polymerase fit together in time at a replication fork?
Which event marks the beginning of DNA replication in a molecule that is about to be copied?
Before a cell divides, every chromosome comes to consist of two identical sister chromatids joined at a centromere. During which part of the cell cycle is the copying that produces them carried out?
A cell is forced into mitosis before it has finished copying its DNA. What is the most likely consequence for the two daughter cells?
On the leading strand, the new DNA is built as one continuous piece rather than as short Okazaki fragments.
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How We Learned DNA Carries the Code
20 questions